I Just Want to Be a Quiet Top Student

Chapter 710 Do not imitate other research teams

The 10 experimental mice in Han Meng’s group performed well in the T maze, and Han Meng will do further research on his friends.

The five experimenters sorted out the raw data of their experimental mice and gave it to Shen Qi.

Shen Qi uses a combination of mathematics, physics, chemistry and biology to conduct an in-depth analysis of the original data.

After the rats in the Duyuan group modified the SHANK3 gene, they all died of intracranial hemorrhage within a short period of time.

Why does Du Yuan’s editing plan cause intracranial hemorrhage in rats?

Regarding this point, Shen Qi didn’t want to understand, he needed to continue to study.

The editing plans of Cui Hualin and Tang Yaxing made their experimental mice become extremely violent or autistic.

Regarding this point, Shen Qi analyzed the general reason.

According to the editing plan of Cui Hualin and Tang Yaxing, after SHANK3 gene modification, it caused new mutations in rat genes in a short time, resulting in irreversible damage to the rat nervous system.

The above three sets of experiments failed.

From the failure, Shen Qi summed up two words: password.

What is the password? where is it? How should it be controlled?

The nucleus contains chromosomes, and the chromosomes are composed of DNA and histones.

DNA was considered a protein molecule in the early days, but it was later confirmed that DNA is actually a deoxyribonucleic acid molecule.

Gene is a DNA sequence that contains genetic information. Its code is genetic information. It expresses the information it carries by producing protein through transcription and translation.

The growth of an organism is caused by successive cell divisions, during which chromosomes are duplicated.

At the same time, if the password is passed on, a new password may also be generated.

Shen Qi believes that the codebook is hidden in the fibrous structure of the chromosome, and the code can be written and read. The key question is, how does the cell choose histones and which way to write and read the code?

In order to master the precise operating principles of biological codes, Shen Qi first studied genes, followed by DNA and proteins, and then chromosomes and cells.

The difficulty in studying genes lies in gene mutations. Shen Qi feels that purely biological methods cannot solve this problem.

τ=Te^W/kT

Shen Qi wrote this formula.

This is an unproven formula, called the “mutation possibility formula” in academia.

Planck discovered quantum theory in 1900, de Vries, Collins, and Chuchermark rediscovered Mendel’s papers in 1900, and de Vries’ papers on mutations published between 1901 and 1903. It can be seen that quantum theory and modern genetics occurred almost simultaneously.

Is there a connection between quantum theory and genetics?

Shen Qi believes that these two disciplines will only be linked when they have developed to a higher level.

Today, quantum theory and genetics have developed to a high degree.

As a result, a new school of thought—quantum biology was born.

Quantum biologists try to explain mutations through quantum theory.

The τ in the sudden change possibility formula is the expected time for the sudden change of the threshold energy W.

The quantum biology school has not yet proven the universality of τ=Te^W/kT, so this formula is temporarily called the “mutation possibility formula” instead of the “mutation formula”.

“The threshold is the order of magnitude needed to explain the persistence of biological heredity…”

Shen Qi condensed his eyebrows and thought, he continued to analyze the experimental data of rats in Han Meng group and Wu Junqiang group.

Before long, Wu Junqiang said with a frown: “Director Shen, there is bad news. The IQ of my laboratory mice has dropped. They can only walk out of the all-right labyrinth, but in other respects, they become like stupid mice. silly.”

Shen Qi didn’t think so: “No, this is not bad news, this is good news.”

Wu Junqiang didn’t understand: “It’s good news that experimental mice have reduced IQ through gene editing?”

Shen Qi expressed his point of view: “IQ is regulated by genetic means. The so-called regulation should be adjusted up or down. This is a complete set of operating procedures. Of course, we are more inclined to increase IQ. But it is undeniable that lowering the academic value of IQ. In some specific circumstances, lowering the IQ of the experimental target may be of greater practical use.”

Wu Junqiang shook his body and said, “Chi attack?”

Shen Qi smiled slightly: “Xiaoqiang, how well do you know τ=Te^W/kT?”

“This is the mutation probability formula proposed by the quantum biology school. I know something about it. My tutors abroad are obsessed with quantum biology. I understand this formula like this…”

After some exchanges, Wu Junqiang suddenly became clear, and he began to draft a thesis.

The title of the paper is “Rat Study of Multiple SHANK3 Gene Editing Methods”.

The summary is as follows: “Studies have shown that the IQ of rodents is affected by the SHANK3 gene to a certain extent. This research group selected Wistar rats as the experimental subjects, and through mismatch editing, nucleotide excision editing, base excision editing, and the same Source recombination editing, non-homologous end joining editing and other methods have been used to edit the SHANK3 gene in experimental rats, and preliminary research progress has been made.”

Four other experimenters also joined the ranks of the paper.

Han Meng wrote in the part of the paper he was in charge of that through the mismatch editing method, the IQ of the experimental rats was significantly improved. And listed the main experimental data, and carried out the reason analysis.

Wu Junqiang wrote in the part of the paper he was responsible for, that the IQ of experimental rats was reduced by the base excision editing method. And listed the main experimental data, and carried out the reason analysis.

Cui Hualin, Tang Yaxing, and Du Yuan also gave their experimental data and experimental results.

Cui Hualin, Tang Yaxing, and Du Yuan were puzzled by the real causes of intracranial hemorrhage, violent, and autism in experimental mice. The analysis of the reasons for this part of the failure lesson is authored by Shen Qi.

Thanks to everyone’s hard work, the “Rat Study of Multiple SHANK3 Gene Editing Methods” was quickly written.

It looks a little hasty, but Shen Qi thinks it can be published first.

There are many types of animal experiments, such as oral or injecting drugs on animals. The experimental cycle is the drug effect cycle and the animal’s metabolic cycle.

In the experiment of gene editing on animals, the experimental cycle should be the normal life cycle of the animal, and even the life cycle of one or several generations of experimental animal progeny should be taken into consideration.

Under normal circumstances, Wistar rats can live for several years.

Then the papers produced by the gene editing experiments on Wistar rats should be completed only a few years later when the Wistar rats die.

The authors of the thesis even have to observe various indicators of the offspring of Wistar rats before they can complete the thesis and give genetic conclusions.

It will take at least five or six years to do this .

Shen Qi couldn’t wait that long, nor did his five PhD students.

Therefore, they wrote in the abstract and summary of the paper: “This experiment has achieved preliminary research results. In the next stage, we will continue to observe various indicators of experimental rats and further study the operating principles of the SHANK3 gene.”

It must be written this way, because the conclusions of the next stage of research may overturn the preliminary conclusions of this paper.

In the preliminary conclusion, the Shen Qi team gave their core views:

1. The SHANK3 gene does affect the IQ of rats to a certain extent;

2. Editing the SHANK3 gene through technical means can adjust the rat’s IQ;

3. There are risks in regulating IQ, and there is a high probability of triggering new mutations, but the mechanism that triggers new mutations has not been fully understood. Before this team gives a final conclusion, other research teams must not imitate.