I Just Want to Be a Quiet Top Student

Chapter 709 Very reliable

Five experimenters entered the observation phase.

The five groups of rats were numbered as Han Meng group, Cui Hualin group, Tang Yaxing group, Du Yuan group, and Wu Junqiang group.

The miracle did not happen for the time being, but the tragedy happened soon.

After the anesthesia effect of the Duyuan group rats ended, they died one day after another.

The experiment record is:

No. 1 mouse in Duyuan group died at 12:42 with intracranial hemorrhage.

No. 2 mouse in Duyuan group died at 12:43 with intracranial hemorrhage.

…

No. 10 mouse in Duyuan group died at 12:51 with intracranial hemorrhage.

To be precise, the 10 experimental mice in Du Yuan’s group died one after another within ten minutes, with an average of one dead per minute.

The cause of death was the same, all died of intracranial hemorrhage.

“Hey, I failed.” Du Yuan was very upset. His experimental plan was proved to be a complete failure. Ten of his friends died suddenly and tragically in the laboratory before they could take the IQ test.

Du Yuan can only be upset for one minute. He must race against time to dissect the 10 dead experimental mice. The main task is to dissect and analyze the brain tissue of the experimental mice, sum up the experience and lessons, and accumulate valuable data for the next experimental design.

There are real risks in conducting gene editing experiments on living organisms.

Du Yuan only modified a gene of the experimental mouse, which caused his experimental mouse to be annihilated in a very short time.

“Brother, don’t be discouraged, failure is the mother of success.” Han Meng comforted Du Yuan, and he said again: “You should sort out the analysis report of your experimental data as soon as possible, and give your conclusion that it is homologous recombination. Is there a problem with the editing method, or is there a problem with your path selection based on this method? We can only take the elimination method now, trial and error one by one, until we find a relatively correct method and specific path.”

While Du Yuan summed up the experience and lessons, the other four experimenters continued to observe the rats in their group.

Soon after, Shen Qi joined the ranks of observers. He noticed that the rats in Cui Hualin’s group became very manic. The rats in this group bite whomever they see. If they can’t bite people, they bite the same kind. If they can’t bite the same kind, they bite the cage. metal net.

“Squeak!”

The rats of Cui Hualin group bared their teeth and looked hideous. Now they don’t like fresh fruits and vegetables. They just want to bite live things.

“Hey, I failed.” Cui Hualin sighed. His little friend was so crazy after gene editing that he couldn’t do an IQ test at all. The craziest rat actually bit its own front foot.

The smell of blood made the rat more crazy. It slammed into the metal net, trying to break through the cage to bite the living thing outside the cage.

Shen Qi patted Cui Hualin on the shoulder: “Extract living cells and brain tissue for preservation, and then put it to death.”

“Director Shen, why don’t we observe for a while?” Cui Hualin was unwilling to fail like this, he still had a glimmer of hope.

Shen Qi shook his head: “You don’t need to continue to observe, put all the experimental mice in your group to death. I want to see their original data. If I didn’t expect it, the modification of their SHANK3 gene affected their neurons. This effect They are irreversible, and I am afraid they will become even crazier.”

“Okay…” Cui Hualin tearfully executed his little friend.

Before execution, Cui Hualin first used aerosol anesthesia to faint his little partner, extract living cells, and then dislocated to death, dissected, processed, and preserved various organs and brain tissues.

There is not a single good news, but bad news one after another.

The rats in the Tang Yaxing group did not show crazy aggressiveness, but they became extremely autistic. They curled up in the corner of the cage motionless, like a still hairy mouse.

“Your group of autistic experimental mice can’t carry out follow-up experiments, extract living cells and brain tissue, save them, and then put them to death. I want to see their original data.” Shen Qi gave instructions.

“It’s a pity, it’s a pity.” Tang Yaxing shook his head and sighed, then executed his little friend.

At present, one group of rats died due to their own causes, and the other two groups of rats with abnormal behavior were executed by the experimenter.

The progress of human science is inseparable from the contributions made by these laboratory animals.

Scientific research continues, and the rats continue to contribute to the advancement of human science in their short lives.

After observation and various physical examinations, the experimental mice in the Han Meng group and Wu Junqiang group were temporarily normal in terms of physical indicators and animal behaviors, and the T maze test can be performed.

I don’t know if it’s good news or bad news. The T-maze test results of the rats in Wu Junqiang’s group remain the same as before.

When this group of rats encountered a T-shaped intersection in the T maze, their choice was always to go to the right.

Most rodents have a one-way preference. The Wistar rats selected by Shen Qi’s team prefer to the right.

The T-maze route designed by Wu Junqiang is eighteen right and one left.

The Wistar rat moves to the right 18 times and then to the left once to get out of the maze and obtain the food placed at the exit of the maze.

The spatial learning ability of the rats in Wu Junqiang’s group has not become stronger, they are quite iron, they have been to the right and never to the left.

In other words, the experimental mice of Wu Junqiang group after SHANK3 gene editing, their IQ remained unchanged, still at a normal level.

“The experimental mice in Wu Junqiang’s group are worth continuing to study.” Shen Qi gave the above instructions.

“Okay, understand!” Wu Junqiang rubbed his hands in excitement. His little friend will not be executed for the time being. His experimental plan has not been proven to be the most suitable plan, but it has not been completely denied.

The five experimenters personally selected their rats, took care of them, and fed them. They had more or less affection for their little friends.

A moment’s life is also life. If you can give your friends a way to survive, even if you live for a few more days, the experimenters don’t want to kill them immediately.

Thirty rats were killed, and ten rats were kept. So far, the rats in the Han Meng group are in the best condition.

Han Meng designed a new T-maze route. The results of his first three experimental mice in the maze were better than those before they received SHANK3 gene editing.

“Han Meng’s group of experimental mice is very hopeful.” Shen Qi finally waited for a piece of good news that seemed good.

“Brother Meng, the rats in this group have improved their learning ability!” Other experimenters watched.

The No. 4 rat in Han Meng’s group entered the T maze. It was a female rat.

Scientists have verified that female rats in this category of Wistar rats are smarter than male rats.

Why female rats are smarter, the essential reason needs to be further confirmed.

But even the clever female Wistar rats their right preference is still obvious.

Before doing gene editing, the female mouse of Han Meng’s group No. 4 could only get out of the T-maze, which is all right.

In this new T-maze, Han Meng set up two left-right T-shaped intersections.

After the first three female rats failed several times, they remembered the correct route and the wrong route. They walked out of the maze and ate food.

Female No. 4 performed even better. It only failed twice and got out of the maze.

The right preference of female No. 4 is still the main characteristic, but at the same time, its learning ability has become significantly stronger.

When it realizes that it can’t get food by going to the right, it will make a change and choose to go to the left at the same intersection.

Cui Hualin tutted amazed: “Mouse No. 4 is quite clever! If it fails once at an intersection, it can remember that the left direction is correct, and choose to go left in the next attempt! Brother Meng, it seems that your gene editing method is very reliable. what!”